64例血脂正常人胆固醇吸收与合成标志物检测

Detecting Cholesterol Absorption and Synthesis Markers in 64 Healthy Persons

目的了解健康成人胆固醇吸收与合成标志物的均值及分布情况。方法入选北京安贞医院体检中心64例健康成人,常规测定肝肾功能、血清总胆固醇、高密度脂蛋白胆固醇、甘油三酯、空腹血糖等指标;采用气相色谱法测定胆固醇吸收与合成标志物。结果 5种非胆固醇类固醇总和平均值为1.02 mg/dL,约为胆固醇总量的0.72%,且存在个体差异;与女性比较,男性血清中7-烯胆甾烷醇水平升高(P<0.05),而豆固醇水平较女性低(P<0.01);合成标志物角鲨烯随着年龄的增长而降低,吸收标志物豆固醇随着年龄的增长而升高。结论通过检测胆固醇吸收与合成标志物,了解健康个体间胆固醇吸收、合成标志物分布情况。

Aim To know means and distribution of cholesterol synthesis and absorption markers in healthy persons. Methods 64 healthy adults were selected in medical examination center of Anzhen hospital and conventional determination of liver and kidney function, serum total cholesterol （TC） , high density lipoprotein cholesterol （ HDLC）, triglyceride （TG）, fasting plasma glucose （FPG）. Serum markers of cholesterol synthesis and absorption were determined with gas chromatogram. Results The sum of the average of five kinds of non-cholesterol steroids was 1.02 mg/ dL, approximately 0. 72% of total cholesterol, and there were individual differences. Serum lathosterol level in male was significantly higher than women （ P 〈 0. 05 ） , while serum stigmasterol in male were significantly lower than women （ P 〈 0. 01 ）. Synthesis markers of squalene decreased with the age increasing and absorption markers of stigmasterol increased with the age increasing. Conclusions It is through the detection of cholesterol absorption and synthesis markers to learn the distribution of cholesterol metabolism markers in healthy individuals.