Bcl-2、血管内皮生长因子在肝癌中的表达及意义

Expression and significance of Bcl-2,vascular endothelial growth factor in liver cancer

目的检测Bcl-2、血管内皮生长因子(VEGF)在肝癌组织、癌旁肝组织及正常肝组织中的表达,进而探讨它们与肝癌的关系。方法采用免疫组织化学染色链霉菌抗生素蛋白-过氧化物酶连结法(SP)法检测在60例肝癌组织标本、30例癌旁标本及14例正常肝标本中Bcl-2、VEGF的表达,并对其临床病理特点进行分析。结果在肝癌组织中Bcl-2、VEGF表达率最高,与正常组织间的表达差异有统计学意义(P<0.05),Bcl-2 75.0%(45/60)vs21.4%(3/14)(P<0.05);VEGF 73.3%(44/60)、21.4%(3/14)。Bcl-2、VEGF的表达在无包膜、有癌检、有淋巴结转移、Ⅲ+Ⅳ分期者中检出率高(P<0.05);VEGF表达在无包膜,肝癌分期Ⅲ+Ⅳ期者中检出率高(P<0.05)。Bcl-2、VEGF阳性表达组患者1年、2年生存率低于阴性组患者(P<0.05)。结论 Bcl-2、VEGF与肝癌生物学特性密切相关,可作为肝癌患者预后的预测因素。

Objective To evaluate expression of Bcl-2 and vascular endothelial growth factor （VEGF） in liver cancer, para-tumor and normal liver tissue, and analyze the relationships between the expression and the clinieopalhologieal characteristics of liver cancer. Methods Immunohistocbemistry was used to detect the expression of Bcl-2 and VEGF in 60 tumor tissues,30 tumor-surrounding tissues and 14 normal liver tissue samples. Log-rank was used to determine the relationship between the expression and elinicopathological parameters of liver cancer. Results Bcl 2,VEGF expression showed the highest rate in hepatocellular carcinoma and the expressions showed significant difference with tumor-adjacent tissues and normal liver tissue （ P 〈0.05）,Bel-2 75.0% （45/60） vs 50.0% （15/30）, 21.4%（3/14）（ P 〈0.05） ;VEGF 73.3%（44/60） vs 43.3G（13/30）,21.4G（3/14）. There were significant differences between Bcl-2,VEGF expression and liver cancer stage,lymph node metastasis,with or without tumor thromhus,with or without envelope formation （ P ~0.05） ; However,there was no difference between expression of bct-2 ,VEGF and gender,age,tumor size, tumor grade and alpha-fetoprotein （AFP） （P i〉 0.05）. Bcl-2 and VEGF showed positive expression in concordance in hepatocellular carcinoma specimens（ P d0.05）. One year and two-year＇s survival ratio in Bcl--2,VEGF positive expression group were significantly lower than those of patients with the negative expressions. The survival time of patients with positive group was significantly lower than that of patients with the negative group（ P〈0.05）. Conclusion Bcl-2, VEGF is closely related to biological characteristics of liver cancer, which is closely related with the prognosis of patients in hepatocellular carcinoma.