桂枝甘草汤乙酸乙酯萃取部位的药效学研究

Separation of Ethyl Acetate Fractions in Ramuli Cinnamomi and Glycyrrhizae Decoction and Their Anti-arrhythmia Efficiency

目的对桂枝甘草汤乙酸乙酯部位进行化学分离并探讨各分离组分的抗心律失常作用。方法对桂枝甘草汤乙酸乙酯萃取所得浸膏,采用AB-8大孔树脂进行分离,得到30%、60%及90%乙醇洗脱物;再采用氯仿诱发的心律失常模型对大孔树脂分离得到的各组分,进行抗心律失常的药效筛选。结果与模型组比较,30%乙醇洗脱组小鼠的心律失常发生率、心率均显著降低(P<0.05),并可缩短小鼠的心律失常持续时间,差异均有统计学意义(P<0.05);与乙酸乙酯部位组、桂枝甘草汤原液组及肉桂酸组比较,其作用效果相当(P>0.05)。结论桂枝甘草汤乙酸乙酯部位的30%乙醇洗脱组分对氯仿致心律失常小鼠有一定的保护作用。

Objective To separate the ethyl acetate fractions in Ramuli Cinnamomi and Glycyrrhizae Decoction and to study their antiarrhythmia efficiency. Methods The AB-8 macroporous resin was applied to the separation of extractum of ethyl acetate fractions. And then chloroform-induced arrhythmia mouse model was established for the screening of antiarrhythmia fractions from 30 %, 60 % and 90 % ethanol eluates. Results In the antiarrhythmic experiment, the group of 30 % ethanol eluate from ethyl acetate fractions through macroporous resin had lower arhythmic incidence and heart rate than the model mice （P 〈 0.05）, and had shorter duration of arrhythmia （P 〈 0.05）. The results also showed that 30 % ethanol eluate from ethyl acetate fractions had the equivalent effect with ethyl acetate fractions, Ramuli Cinnamomi and Glycyrrhizae Decoction, and cinnamic acid（P 〉 0.05 ）. Conclusion The 30% ethanol eluate from ethyl acetate fractions through macroporous resin shows certain anti-arrhythmia efficiency on chloroform-induced arrhythmia mouse.