微量元素肥料和茉莉酸甲酯处理对金银花有效成分含量的影响

Influences of micronutrient fertilizer and methyl jasmonate treatments on the content of compounds in Lonicera japonica

目的:应用高效液相色谱法(HPLC),研究不同微量元素肥料(微肥)、不同浓度茉莉酸甲酯(MeJA)处理对金银花中7种有效成分含量的影响。方法:应用转换波长的高效液相色谱分析方法,色谱柱为Agilent Eclipse Plus C18柱(250mm×4.6mm,5μm);流动相为0.3%甲酸水溶液(A)和乙腈(B),进行梯度洗脱;流速1mL/min;采用VWD紫外检测器转换波长(330、350nm)检测;柱温为常温。结果:不同微肥、不同浓度MeJA处理能不同程度地影响金银花中各个成分或总成分含量。结论:不同微肥处理中,Fe处理可同时增加绿原酸、木犀草苷含量;不同浓度MeJA处理中,浓度为800、1 600μmol/L的MeJA可增加绿原酸及总成分含量。

Objective： A high-performance liquid chromatographic（HPLC） method was developed to research the influences of micro-elements and methyl jasmonate treatments at the content of 7 kinds of compounds in Lonicera japonica. Methods： HPLC was developed to determine the contents of seven compounds in Lonicera japonica and folium lonicerae with wavelength conversion, analysed the content differences between them. Chromatographic column was a C18（250mm × 4.6mm, 5 μ m） column operating at normal temperature with a flow rate of lmL/min, and detection wavelength at 330, 350nm. The mobile phase using gradient flow consisted of two solvent systems： 0.3% methanoic acid and acetonitrile. Result： Different kinds of micro-elements and different concentration of methyl jasmonate had an certain impact on each or total content of compound in Lonicera japonica. Conclusion： In treatments of different kinds of micro-elements, Fe treatment can simultaneously increase the contents of chlorogcnic acid and luteoloside; in treatments of different concentrations of methyl jasmonate, concentration of 800μmol/L, 1 600μmol/L can increase the contents of chlorogcnic acid and total contents.