摘要
目的:对重症监护病房(ICU)出现的13株耐亚胺培南肺炎克雷伯菌的分子流行病学及耐药机制进行研究。方法:从ICU分离到13株对亚胺培南耐药肺炎克雷伯菌,采用琼脂稀释法检测包括亚胺培南在内的5种抗菌药物最低抑菌浓度(MIC);用聚合酶链反应(PCR)及耐药基因克隆测序方法研究细菌的耐药机制;用肠杆菌基因间重复性一致性序列-PCR分析菌株的分子流行病学。结果:13株肺炎克雷伯菌亚胺培南的MIC为16~1 024μg/ml;13株实验菌PCR产物克隆测序分析比对为blaKPC-2型和blaSHV型基因。结论:ICU连续出现13株耐亚胺培南肺炎克雷伯菌,KPC-2是引起细菌对亚胺培南耐药的主要原因,SHV型β-内酰胺酶同时参与多重耐药机制。
Objective:To investigate the molecular epidemiology and mechanism of carbapenem resistance of Klebsiella pneumoniae from intensive care units (ICU). Methods:Thirteen strains of Klebsiella pneumoniae with carbapenem resistance were isolated from ICU. The minimum inhibitory concentrations(M1C) were detected by using agar-dilution, polymerase chain reaction(PCR) and cloning and sequencing of resistance genes were used to analyzed the resistant mechanism. Enterobacterial repetitive intergenic consensus-PCR was performed to analyze the molecular epidemiology. Results:The MICs of imipenem of 13 strains of KlebsieUa pneumoniae were from 16 to 1 024 μg/ml, which were blaKPC-2 and blaSHV using the PCR product cloning and sequencing method. Conclusions: Carbapenemase blaKPC-2 is the major factor of carbapenem resistance, and laetamase blaSHV participates multiple mechanisms of resistance.
出处
《蚌埠医学院学报》
CAS
2013年第1期99-102,共4页
Journal of Bengbu Medical College
基金
安徽省淮北市科技局自然科学资金资助项目(00242)
关键词
肺炎克雷伯杆菌
耐药
重症监护病房
亚胺培南
Klebsiella pneumoniae
drug resistant
intensive care units
carbapenem