洗涤液温度对自体血回收红细胞回收率的影响

A study about the effect that the temperature of the washing liquid makes on red blood cell recovery rate of Autologous Blood Recovery

目的比较不同温度生理盐水(NS)对神经外科术中自体血液回收红细胞回收率的影响。方法采用自体血液回收的神经外科手术患者60例,根据洗涤液NS温度不同,随机分为室温24℃组(S组)和控温4℃组(D组),每组30例。分别记录洗涤前后的回收血量,测定两组清洗前后回收血液ph值,Na+,K+,血红蛋白(Hb),红细胞压积(Hct)。取两组清洗后血液,S组常温放置,D组放于4℃冰箱内,测定两组血样放置1h,2h和4h后回收血液pH,Na+,K+,Hct,Hb。结果清洗前,两组间血液各项指标均无明显统计学差异(P>0.05),清洗后两组血液pH,Na+和Hct,Hb均明显高于清洗前(P<0.01),且K+明显降低(P<0.01)。计算红细胞回收率(Sr),D组高于S组(P<0.01)。结论采用4℃NS作为洗涤液并且以4℃存储回收处理过的血液,可以更好的保护回收血液的红细胞,提高回收血液质量。

Objective compare the effects that different temperature Normal saline（NS）on erythrocyte recoveries in autologous blood recovery during cerebral surgery. Methods Autologous blood recovery of cerebral surgery with 60 cases, according to the different temperature of NS, randomly divided into room temperature 24℃（group S） and temperature 4 ℃ groups （group D） ,30 cases in each group. Record the recovery of blood volume respectively before and after washing,Test recovery blood ph value, Na＋ , K＋ , hemoglobin （Hb）, hematocrit （Hct）, Take two groups after cleaning blood, group S placed in indoor temperature,group D on the refrigerator of 4℃. Test blood samples that have been placed for 1 hour, 2 hours and ,4 hours, get blood ph,Na＋ ,K＋ , Hct, Hb. Results before cleaning the blood, there was no significant statistical difference be- tween the two groups （P〉0.05）, after cleaning, two sets of blood ph, Na＋ and Hot, Hb were significantly higher than that be fore washing（P〈0.01） ,and K＋ was obviously decreased（P〈0.01）. Calculated red blood cell recovery rate （Sr）, D group is higher than S group（P〈0.01）. Conclusion the 4℃ NS as a washing liquid and Stored in the 4℃ environment,can better pro- tect recovery blood red blood cells,improve the quality of blood reeovery.