自然越冬过程中山葡萄抗寒生理指标的变化

Change of Cold-resistant Physiological Indicators of Vitis amurensis During Natural Overwintering

为了探明山葡萄扦插苗的抗寒生理特性,以二年生山葡萄扦插苗枝条为材料,在自然越冬过程中,研究山葡萄枝条组织的含水量、可溶性糖、游离脯氨酸、可溶性蛋白含量及细胞膜透性的变化。结果表明:山葡萄扦插苗在自然越冬过程中,其扦插苗枝条的各种抗寒生理指标变化均存在一定差异,含水率在2011年11月—2012年3月的变化不大,4月随着气温的升高,枝条中的含水量升高,含水率达84.5%,且与其他各测定时间的差异达显著水平;可溶性糖、可溶性蛋白、游离脯氨酸含量和相对电导率随温度的变化呈先上升后降低的趋势,但不同生理指标的变化幅度存在一定差异。

To explore cold-resistant physiological indicators of V. amurensis cutting seedlings, taking 2-year-old V. amurensis cutting seedlings as the material, the contents of tissue water, soluble sugar, free proline and soluble protein in V. amurensis branches and the change in cell membrane permeability were studied. The results showed that in the process of natural overwintering the changes of various indicators had some difference. The moisture content was stable from Nov. 2011 to Mar. 2012, and increased with temperature increasing in April. The peak value was 84. 5%, which was significantly different with others. The contents of soluble sugar, soluble protein, free proline and relative conductivity first increased and then decreased with temperature rising. However, the rangeability was significantly different between each two of the physiological indicators.