草乌甲素对大鼠佐剂性关节炎的影响

Effect of Bulleyaconitine A(BLA) on Adjuvant Arthritis Rats

目的:了解草乌甲素对佐剂性关节炎(AA)大鼠原发性关节炎的抗炎镇痛作用。方法:用Freund's完全佐剂免疫Wistar大鼠建立AA动物模型,将其随机分为3组,即草乌甲素治疗2组(剂量分别为100μg.kg-1、50μg.kg-1)、注射用水组。于AA动物模型建立当天对实验大鼠进行处理,连续7d,并记录各组大鼠体重、右踝关节周径变化。治疗结束当天记录炎侧踝关节屈关节疼痛试验评分,用ELISA法检测大鼠血清的TNF-α、INF-γ、PGE2、β-内啡肽含量。结果:在100μg.kg-1治疗组中,草乌甲素在治疗第2天即可明显减轻关节肿胀(P<0.05),提高AA大鼠痛阈(p<0.05),显著降低AA大鼠PGE2含量、提升血清β-内啡肽含量(P<0.01);在50μg.kg-1治疗组中,草乌甲素从治疗的第3天起开始减轻关节肿胀(P<0.05),对大鼠PGE2含量、提升血清β-内啡肽含量作用较弱(P>0.05)。100μg.kg-1、50μg.kg-1草乌甲素均可降低AA大鼠血清TNF-α含量(P<0.01),但两者间无统计学差异;草乌甲素对血清INF-γ无明显影响(P>0.05)。结论:草乌甲素明显改善AA大鼠关节的肿胀,这可能与其降低血清TNF-α、PGE2含量有关;草乌甲素提高AA大鼠痛阈可能与降低血清PGE2,提升血清β-内啡肽有关。

Objective：To evaluate the effect of bulleyaconitine A（BLA） on adjuvant arthritis rats.Method：AA Wistar rats were induced by Freund＇s complete adjuvant,Beginning from the onset of AA,AA Wistar rats were randomly assigned to bulleyaconitine A（at a dose of 50μg/kg（n=12）,or 100μg/kg（n=12））,or Aqua pro injection（at a dose of 100μL,n=12）,once daily,respectively,for seven days.Increased percentage of body weight,change of circumference of ancle,and scores of arthritic flexion pain test were evaluated.The levels of TNF-α,INF-γ,PGE2 and β-EP were measured with the use of ELISA.Result：A 100μg/kg of BLA can significantly reduce the circumference of arthritic ankle starting from the 2nd day（p0.05）,and the same result occurred at 50μg/kg of BLA from the 3rd day（p0.05）.BLA produced significant analgesia at a dose of 100μg/kg（p0.05）.The levels of TNF-α and PGE2 increased in 100μg/kg BLA treated rats（p0.01）.The levels of TNF-α in rats treated with 50μg/kg BLA decreased significantly as compared with Aqua pro injection group（p0.01）,and there was no difference between 50μg/kg group and 100μg/kg group.Conclusion：BLA can improve the inflammation of AA rats,which was correlated with its ability of reducing the TNF-α and PGE2 levels in serum.BLA can also reduce inflammatory pain by reducing the level of PGE2 and increasing the level of β-EP in serum.