清解防感颗粒体内外抑菌作用实验研究

Antibacterial Effect of Qingjie Fanggan Particles in Vivo and in Vitro

目的 :研究清解防感颗粒的体内外抑菌作用。方法 :采用标准试管二倍稀释法测定清解防感颗粒的最低抑菌浓度(MIC)和平板转种法测定最低杀菌浓度(MBC),并观察其在小鼠体内外对金黄色葡萄球菌、表皮葡萄球菌和乙型溶血性链球菌的抑菌作用。结果 :体外抑菌试验显示:清解防感颗粒对上呼吸道常见细菌有不同程度的抑菌作用,其中对金黄色葡萄球菌、表皮葡萄球菌和乙型溶血性链球菌的作用较强,对铜绿假单孢菌和白色念珠菌也有一定的抑制作用。体内抑菌作用显示:清解防感颗粒8、4 g/kg剂量组有抑菌作用,可明显降低金黄色葡萄球菌感染小鼠的死亡率并延长生存期。结论 :清解防感颗粒具有一定的体内外抑菌作用。

Objective ： To study the antibiotic vitro. Method： The experiment adopted standard activities of Qingjie Fanggan Particles in vivo and in tube double dilution method to measure the Minimal Inhibitory Concentration （ MIC ） of Qingjie Fanggan Particles and used the flat turn method to measure minimum bactericidal Concentration （ MBC ）, observing its antibacterial effect on staphylococcus aureus, staphylococcus epidermidis and betahemolytic streptococcus in mice. Results ： Antibiotic experiment in vitro showed that Qingjie Fanggan Particles had antibiotic effect in varying degrees on common bacterium of upper respiratory tract, especially on staphylococcus aureus, staphylococcus epidermidis and beta hemolytic streptococcus. Meanwhile, it also had inhibitory effect on pseudomonas aeruginosa and candida alicans in some degree. Antibacterial effect in vivo showed that the group infected by staphylococcus aureus which was given 8 g/kg and 4 g/kg of Qingjie Fanggan Particles had conspicuously reduced the mortality and prolonged survival period. Conclusion： Qingjie Fanggan Particles has some antibiotic activities in vivo and in vitro.