益母草饮片中盐酸水苏碱、盐酸益母草碱含量测定

Content Determination of Hydrochloric Stachydrine and Hydrochloric Leonurine in Decoction Pieces of Motherwort

目的：建立益母草饮片中盐酸水苏碱、盐酸益母草碱的含量测定方法，为其质量控制提供依据。方法：采用高效液相色谱法对益母草饮片中盐酸水苏碱的含量进行测定，色谱柱：SCX强阳离子柱（4．6HunX250mm，5μm）；流动相：0．5mol磷酸二氢钾-三乙胺（1000：1．5）（磷酸调pH值2．25～2．3）；检测波长：192nm；流速1．0mL·min-1柱温：25℃。结果：盐酸水苏碱的浓度在0．2～2．0mg·mL-1范围内线性关系良好。IKSD=1．7％（n=5）：盐酸益母草碱的浓度在0．2～2．0mg·mL-1范围内线性关系良好，RSD=0．08％（n=5）。结论：该法简便、灵敏、准确，可用于益母草饮片的质量控制。

Objective ： To establish a method for content determination of hydrochloric stachydrine and hydrochloric leonurine in decoction pieces of motherwort, and to provide the basis for its quality control. Methods ： An HPLC method was established by using an SCX strong cation exchange column （4.6 mm × 250 mm, 5μ m） with the mobile phase of 0.5 mol monopotassium phosphate ： triethylamine（1000 ： 1.5）, phosphoric acid adjusting the pH value to 2.25～2.3, with the flow rate of 1 mL ·rain-1, the wavelength of 192 nm and column temperature of 25 ℃ . Results ： There was good linear relationship when the concentrations of hydrochloride stachydrine and hydrochloric leonurine were 0.2～2 mg· mL-l and the values of RSD were 1.7%（n=5） and 0.08%（n=5） respectively. Conclusion ： The method is simple, sensitive and accurate, which can he used for the quality control of motherwort herb pieces.