广西地区乙肝病毒基因分型与临床表现及机体免疫功能关系的研究

Association between the HBV Genotypes and Clinical Manifestations of HBV Relative Liver Diseases and Immune Status

目的:研究广西地区乙肝病毒基因型分布及与临床表现及机体免疫功能的关系。方法:收集广西地区原籍人口不同病情的慢性乙肝病毒相关性疾病包括无症状携带者(ASC)、慢性乙型病毒性肝炎(CHB)、乙肝后肝硬化(LC)、原发性肝癌(HCC)血液标本,检测乙肝病毒血清学标志物(HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc和抗-HBcIgM),HBVDNA拷贝数,肝功能,T淋巴细胞亚群,型特异性引物巢式PCR及测序鉴定病毒基因型,利用统计软件SPSS17.0进行资料分析。结果:本研究检测纳入的研究对象感染乙肝病毒为B、C两种基因型,未检测到其他基因型,以C型(66.3%)为主,B型(33.7%)次之。C基因型的HBeAg血清转换率低于B型(P<0.05)。C型在肝癌组的比例显著高于其他疾病组(P<0.05),不同基因型间年龄、HBVDNA拷贝数、AST、ALT、T细胞亚群CD3(+)、CD4(+)、CD8(+)的差异无统计学意义。结论:广西地区乙肝病毒感染相关性肝病患者HBV基因型为B型、C型,以C型为主,C型与较严重病情有关,感染不同HBV基因型与机体的免疫状况无明显相关。

Objective ： To investigate the distribution and virologic features of HBV genotypes in GuangXi province and the association of the clinical manifestation.Method： Serum samples were collected from asymptomatic carrier （ ASC ）, liver cirrhosis （ LC ）, chronic hepatitis B （ CHB ）, primary hepatic cell carcinoma （ HCC ） individuals. Liver function, biochemical markers and T-lymphcyte subpopulations were detected.Type-specific primers nested polymerase chain reaction was applied for HBV genotyping. The data was analyzed with SPSS 17.0.Result： Genotype C was found to be predominant （ 66.3% ） among the studied samples, followed by genotype B （ 33.7% ） .HBeAg serum conversion rate of C genetype was significantly lower than that of B genotype, while the proportion of C genotype in HCC group exhibited significantly higher than that in ASC, CHB and LC groups.No significant difference was found in age, viral leads, ALT, AST and T cell subsets between B, C genotypes.Conclusion： There are B and C genotypes prevalent in GuangXi, with C being significantly dominant. Genotype C is associated with more severe liver diseases. The association between the virus genotype and the cellular immunity remain a field to be explored.