用RP-HPLC法测定靶控输注下血浆中的丙泊酚浓度

Determination of propofol concentration in human plasma administered with target-controlled infusion by RP-HPLC method

目的:建立测定靶控输注下血浆中丙泊酚浓度的方法。方法:采用RP-HPLC法,色谱柱:Shim-pack CLCODS-C18柱;流动相:乙腈∶水=65∶35,流速:1.5ml/min;检测波长:220nm;柱温:25℃。结果:丙泊酚在0.05～5.0μg/ml范围内线性关系良好(r=0.999 8),精密度和稳定性良好,低、中、高浓度(0.5、2.0、5.0μg/ml)丙泊酚的回收率分别为(103.0±2.5)%、(98.0±1.4)%和(102.5±3.1)%(n=3)。结论:该方法准确、灵敏、快捷,可用于测定靶控输注下血浆中的丙泊酚浓度。

Objective： To establish a method to determine propofol concentration in human plasma administered with target-controlled infusion.Methods： RP-HPLC method with ultraviolet detector was applied in our study.Separation was performed on a Shim-pack CLC ODS-C18 column with the mobile phase consisting of acetonitrile∶water=65∶35 at a flow rate of 1.5 ml/min.The detection wavelength was 220 nm and the column temperature was 25 ℃.Results： Propofol was shown to be in good linearity within the range of 0.05-5.0 μg/ml（r=0.999 8）,with good accuracy and stability.Recovery rates at low,medium and high concentrations（0.5,2.0,5.0 μg/ml） of propofol were（103.0±2.5）%,（98.0±1.4）% and（102.5±3.1）%（n=3）,respectively.Conclusion： The method is accurate,sensitive and rapid for the determination of propofol concentration in human plasma administered by target-controlled infusion.