面神经损伤后穴位针刺对NGF mRNA表达的影响

Effects of Acupuncture on mRNA Expression for NGF after Facial Nerve Trauma

本文观察了穴位电针刺激对面神经再生过程表情肌、神经及神经核中NGF表达的影响。将日本大耳白兔面神经压榨伤后 ,电针刺激“翳风”、“颧”、“地仓”、“颊车”、“四白”、“阳白”、“合谷”穴 ,每天 3 0分钟 ,2周为 1个疗程。经 1、2、3疗程治疗后 ,应用原位杂交及RT PCR技术检测针刺组及对照组表情肌、面神经及面神经核中NGFmRNA表达水平的变化。结果表明 ,两组动物的三种组织中 ,NGF表达高峰均在神经损伤后第六周 ;在表情肌组织中 ,针刺组三个时象点的NGFmRNA的表达明显高于对照组 (P <0 .0 1 ) ;在面神经及面神经核组织中 ,针刺组原位杂交信号显著强于对照组 ,但RT PCR检测结果在两组中无显著差异 (P >0 .0 5 )。提示 ,在面神经再生过程中 ,穴位电针刺激能明显增强表情肌组织中NGF的表达 ,这对面神经再生可能起促进作用。

Both right and left facial nerve of rabbits were crushed and the degree of the injury was Sunderland Ⅳ. Then the injuried rabbits were devided into treatment and control group. One week after injury, pulse electroacupuncture stimulations at acupoints(PEASA) were performed in the treatment group,while the control group was left recovering naturally. The acupoints included “Yifeng',“Quanliao',“Jiache',“Dicang',“Sibai',“Yangbai' and “Hegu'.Two weeks was a course of the treatment.In this experimental model,we have showed facial nerve regeneration had been improved by acupointpuncture pathologically and electro physiologically.But on molecular level it has been a little known how about the effects of acupuncture on neurotrophic factors during the nerve regeneration. When the first(3w),second(6w),and third(9w)course of the treatment were finished we used in situ hybridization and reverse transcription PCR technique to observe the changes of mRNA expression of NGF in facial nucleus,facial nerve and mimetic muscle tissue.The results were as follows∶(1) mRNA expression of NGF reached the highest level in the sixth week posttrauma in both treatment and control groups;(2) In mimetic muscle tissue,both in situ and RT PCR technique showed expressional level of mRNA for NGF in the treatment group was significantly higher than that in the control group(P< 0.01 ).In nerve and nerve nucleus tissue,only in situ technique showed the sign of NGF was stronger in the treatment group than that in the control group.This experiment result suggests some molecular mechanism taking part in the effects of acupuncture.