医用抛射剂HFC-134a的急性毒性研究

Acute toxicity studies on medical propellant HFC-134a

目的:研究国产医用抛射剂HFC-134a的急性毒性,为进一步扩大其在医疗上的应用提供可靠的实验安全数据。方3法:将60只昆明小鼠分成5组,每组12只,雌雄各半,分设剂量为0、100、500、1000、5000mg/m,连续吸入HFC-134a气体染毒4h,后常规饲料喂养,期间每天观察小鼠行为学改变,7d后处死小鼠,比较染毒前后小鼠体质量变化,并行大体病理形态学检查,观察肝、肾、胰腺等脏器的变化。结果:与阴性对照组相比,染毒组小鼠体质量,行为及肝、肾、胰腺等脏器未发现异常3改变。最高给药剂量(5000mg/m吸入4h)组,无动物死亡,平均体质量增至(26.3±5.8)g,无致炎、致敏和中毒反应。结论:在本实验条件下,医用抛射剂HFC-134a未发现明显的急性毒副反应,常规用药剂量安全。

OBJECTIVE： To investigate the toxicity of medical propellant HFC-134a, in order to provide scientific basis for promoting the application of HFC-134a on medicine. METHODS： 60 Kunming mice were divided into 5 groups, 12 mice each group, male and female in half： 0, 100, 500, 1 000, 5 000 mg/m3. The acute toxicity was conducted with mice exposed for 4 hours, to observe the change of animal behavior. Then all mice were sacrificed after 7 days. The change of mice weight and the pathomorphology of liver, kidney and pancreas were observed. RESULTS： Compared with negative control group, the weight of liver, kidney and pancreas did not show any abnormality. No animal died under the maximum dose. The average weight increase was （26.3 ± 5.8） g, and there was no irritation or toxicity found through respiratory tract contamination. CONCLUSION： There were no obvious acute toxicity and side effects on the experimental subjects. HFC-134a could be used as a medicine safely. [KEY WORDS1 HFC-134a; propellant; LC50; acute toxicity