硫贲妥钠诱导c-fos基因在中枢神经系统的表达

Epression and distribution of c-fos oncogene within central nervous sysyem of the rat following sodium pentothal anesthesia

目的 了解硫贲妥钠麻醉在中枢神经系统的作用部位 ,为进一步探讨全身麻醉作用机制打下基础。方法 用SD大鼠 2 4只 ,分别从静脉注射硫贲妥钠 15、2 0和 2 5mg·kg-1麻醉 1h ,应用c fos基因免疫组织化学法 ,光镜观察Fos蛋白阳性神经元在大脑的分布并计数。结果 研究表明硫贲妥钠麻醉在大脑有 15个Fos阳性神经核团参与麻醉调控作用 ,分别是梨状皮层、伏核、杏仁基底外侧核、外侧缰核、弧束核、外侧僵核、下丘脑室旁核、丘脑室旁核、膝状体腹下核、背外侧膝状核 ,视上核、视交叉上核、视前腹侧核、海马回嗅觉小岛和乳头状核。结论 硫贲妥钠麻醉在中枢神经系统具有明确的作用位点。

AIM To understand the distribution of c fos oncogene expression within central nervous system(CNS) following sodium pentothal anesthesia. METHOD c fos oncogene immunohistochemical technique (ABC method) was employed. RESULTS When anesthetic dose of sodium pentothal was 15 mg·kg -1 ,20 mg·kg -1 or 25 mg·kg -1 , fifteen, fos like immunoreactive neurons(FLNs) appeared in the CNS, including piriform cortex (pir), accumbens nucleus(ACB), basolateral amygdaloid nucleus(BL), lateral septal nucleus(LS),nucleus of the solitary tract(Sol), lateral habenular nucleus(LHb), periventericular hypothealamic nucleus(Pe), paraventricular thalamic nucleus(PV),ventral lateral geniculate nucleus(VLG),dorsal endopiriform nucleus(DEn),supraoptic nucleus(So),suprachiasmatic nucleus( Sch), Anteroventral preoptic nucleus (AVPO),islands of Calleja(ICj),supramammillary nucleus(SuM). CONCLUSION Sodium pentothal anesthesia has the determined sites of action in CNS.