绞股蓝皂苷对小鼠白血病P388细胞内原癌基因表达的影响

Effect of gypenosides on the expression of oncogenes in P 388 cells

目的 研究绞股蓝皂苷 (gypenosides ,GP)体外给药对小鼠白血病P 388细胞内原癌基因c fos和c myc基因表达的影响。方法 采用Northern杂交和狭线杂交的方法检测c fos和c myc基因表达的水平。 结果 GP在给药剂量0 0 15和 0 0 30 g·L-1的条件下 ,体外给药 2 4h ,c fos和c myc的表达水平分别为 0 5 2 2 ,0 947和 0 16 5 ,0 12 0 ,而对照组则为 0 36 0和 0 30 8。结果表明 ,GP作用 2 4h使P 388细胞内的c fos基因表达水平明显升高 ,c myc基因的表达水平明显降低。结论 GP对原癌基因c fos,c myc表达水平的影响是其抑制P 388细胞生长的机制之一。

AIM To study the effect of gypenosides(GP)on the expression of proto oncogene c fos and c myc in P 388 cells in vitro METHODS The levels of c fos and c myc gene expression were determined by Northern blot and slot blot RESULTS The levels of c fos, c myc mRNA in P 388 cells were 0 522, 0 947 and 0 165, 0 120, respectively after 24 h dealt with GP 0 015 and 0 030 g·L -1 , and the levels of gene expression of control group were 0 360 and 0 308 respectively after 24 h in the same condition The experiment showed that the expression of c fos in P 388 cells was increased and that of c myc in P 388 cells was decreased after administration of GP CONCLUSION The effect of GP on the expression of oncogene c myc and c fos is one of the mechanisms of its inhibiting P 388 cells growth