白介素-1受体相关激酶-2反义寡核苷酸对白介素-1和肿瘤坏死因子激活核因子-κB的不同影响

Different responses of IL-1-and TNF-stimulated NF-κB activation to antisense IRAK-2 oligonucleotides

目的 研究白介素 1受体相关激酶 2 (IRAK 2 )在白介素 1(IL 1)与肿瘤坏死因子 (TNF)激活核因子 κB(NF κB)过程中的作用。方法 IRAK 2反义寡核苷酸转染人胚肾细胞 (2 93细胞 ) ,阻断IRAK 2的表达 ,用IL 1及肿瘤坏死因子刺激细胞后 ,用夹心ELISA方法检测NF κB活性。结果 以IRAK 2反义寡核苷酸预处理可明显减弱IL 1诱导的NF κB活性 ,此效应强度存在时间和浓度依赖性 ,但I RAK 2反义寡核苷酸对肿瘤坏死因子诱导的NF κB活性无影响。结论 IRAK 2反义寡核苷酸对IL 1和TNF激活NF κB有不同影响。IRAK 2在IL 1诱导的NF

AIM To investigate the role of interleukin 1 ( IL 1 ) receptor associated kinase 2 (IRAK 2) in IL 1 and TNF stimulated nuclear factor kappa B ( NF κB ) activation METHODS The 293 cell was transfected with antisense IRAK 2 oligonucleotide (IRAK 2 ODN) followed by stimulating the cell with IL 1 or tumor necrosis factor (TNF), and then the levels of NF κB activation was analyzed by sandwich enzyme linked immunosorbent assay (ELISA) RESULTS Pre transfecting with antisense IRAK 2 ODN could remarkably decrease the levels of NF κB activation stimulated by IL 1 in time and concentration dependent manner,and IRAK 2ODN not attenuate the one stimulated by TNF CONCLUSION The responses of IL 1 and TNF stimulated NF κB activation to antisense IRAK 2 oligonucleotids are different IRAK 2 plays a key role in the IL 1 signaling events leading to NF κB activation