不同药物抗风疹病毒的实验研究

STUDY ON ANTI-RUBELLA VIRUS ACTIONS OF DIFFERENT DRUGS

目的 :为筛选有效的抗RV药物。方法 :选用 9种新合成的腺嘌呤衍生物、6种尿嘧啶衍生物及大黄醇提液 ,分别在BHK2 1、RNC和HNC细胞上进行了体外抗病毒试验。在BHK2 1上以CPE为观察指标 ,在RNC和HNC上因不出现明显的CPE ,则将细胞培养物转种于BHK2 1细胞并用间接免疫荧光和免疫酶技术检测药物的抗病毒效应。结果 :取代基为—CH2 —C6H5、— (CH2 ) 2 OH、—C6H5—CH3 的腺嘌呤衍生物及取代基为—CH3、— (CH2 ) 2 OH、—C6H5—CH3、—C6H5—Cl的尿嘧啶衍生物 ,对RVJR 2 3的最小抑毒剂量在 78～ 62 5mg/L之间 ;大黄醇提液对RVJR 2 3的最小抑毒剂量为 50 0 0mg/L。 结论 :上述药物均有抗RV作用 ,大黄醇提液的作用较新合成的部分腺嘌呤与尿嘧啶衍生物更为明显 。

Objective:To find new effective antirubella virus drugs.Methods:Antirubella virus actions of synthesized adenine and uridine derivants and ethanol extract of rhubarb were studied in three kinds of cell cultures.CPE was used as observation index in BHK 21 cell culture.In view of no obvious CPE in primary rabbit cerebral cell cultures and that of human foetus,the antiviral actions of rhubarb in RNC and HNC were examined with transinoculating to BHK 21 cell,indirect fluorescence and enzyme immunological technique.Results:The active replacement radicals for adenine and uridine derivants are—CH 2—C 6H 5、—(CH 2) 2OH and —C 6H 5—CH 3 in Derivant A and —CH 3,—(CH 2) 2OH,—C 6H 5—CH 3 and —C 6H 5P—Cl in Derivant U,their minimum inhibition doses to RV JR23 range from 78 to 625mg/L.For ethanol extract of rhubarb,the minimum inhibitory dose to RV JR23 is 5000mg/L.Conclusion:All drugs mentioned above exhibited antiviral action.Ethanol extract of rhubarb showed more obvious than synthesized adenine and uridine derivants,but did not show cytotoxicity.