组织工程骨-软骨复合组织体内异位移植的研究

Study on heterotopic transplantation of tissue engineering osteochondral composites

目的:本实验构建组织工程骨软骨复合组织并采用异体异位移植方式,探讨外来因素对移植物的影响,为进一步研究奠定基础。方法:制备明胶-硫酸软骨素-透明质酸及明胶-陶瓷化骨多孔复合支架,复合软骨细胞和骨髓间充质干细胞,将上述组织按照体外培养时间不同分为4组,植入裸鼠皮下,观察复合组织形成情况。结果:各实验组在观察期内裸鼠背部均无感染,复合组织表面完整薄层纤维层下可见类似透明样软骨组织,体积均出现不同程度的收缩。HE染色各实验组均可发现,在软骨层和骨层,分别可见成软骨细胞、软骨细胞、骨细胞及相应的细胞外基质,且可以观察到类似正常结构的潮线出现,交界区软骨组织出现骨化,在组织外围,出现了不同程度向纤维样组织转变的现象。结论:经过体外短时间的诱导使得细胞具有分化趋势后,早期植入体内有助于组织工程骨-软骨复合组织的形成。

Objective To construct tissue engineering osteochondral composite tissue,and undergo allogeneic transplantation. Discusses the influence of the external factors on the graft, and establish the foundation for the further research. Methods Gelatin-chondroitin sulfate-hyaluronic acid-gelatin-ceramic osteochondral composites scaffolds were ontracted, an~ seeded with chondrocyte and bone mesenchymal stem cells. Transplanted subcutaneously into nude mice according to different time and then analyzed histogenesis after the operation. Results There was no infection on dorsum in each group during observation period. It could be seen hyaline cartilage like tissue on the surface of composite tissue surface. The volume appeared smaller than pre-operation. HE stain showed cartilage cells cells, osteocyte and extracellular matrix respectively in cartilage and bone layer.It also can be seen tide line similar as nature tissue. In junctional area cartilage appeared ossification, and in peripheral tissue it appeared fibrosis. Conclusion It can be concluded that after short time induced in vitro,the cells have differentiation trend, it will be benefit for forming osteochondral composite tissue.