斑点杂交法检测正畸大鼠三叉神经节PPTAmRNA的变化

Changes in the Expression of PPTA mRNA in the Rat trigeminal Ganglion during Tooth Movement using Dot-blot hybridization method

目的:观察正畸牙齿移动不同时期大鼠三叉神经节(Tri gemi nal Gangl i on,TG)中前速激肽原A(PPTA)mRNA的表达变化,初步探讨牙齿移动导致疼痛的可能机制。方法:采用斑点杂交的方法检测正畸加力后不同时期大鼠TG内PPTAmRNA水平的变化情况。结果:加力2h大鼠三叉神经节PPTA mRNA的表达量开始增加,加力8h至加力3天达到最高,至第7天PPTA mRNA水平仍然明显高于对照组。结论:正畸牙齿移动能导致TG内PPTAmRNA表达水平的上调,提示PPTA可能参与了正畸疼痛的发生。

Objectives To investigate the changes in the Expression of PPTA mRNA in the Rat trigeminal Ganglion during Tooth Movement. Methods The changes in the expression of PPTA mRNA in the rat trigeminal ganglion,during tooth movement in different periods,were detected using Dot-blot hybridization. Results The expression of PPTA mRNA in trigeminal ganglion was stonger than that in blank group and control group at all the time points; PPTA mRNA level was increased after 2 hours and reached the peak level around 8 hours to 3 days during tooth movement,7days after tooth movement PPTA mRNA level was still higher than in control group. Conclusion Orthodontic tooth movement can lead to a stonger expression of PPTA mRNA in trigeminal ganglion, suggesting that the PPTA may be involved in the occurrence of orthodontic pain.