摘要
目的:筛选并分析喉癌组织与周围正常喉黏膜的微小RNA(microRNAs,miRNAs)之间的表达谱差异,为进一步研究miRNA与喉癌发生、发展的关系提供线索。方法:收集喉癌组织和癌旁正常喉黏膜标本共42对,随机选取10对标本进行miRNA微阵列基因芯片分析,另选取32对标本进行实时荧光定量PCR(qRT-PCR)验证,获得喉癌组织中的miRNA差异表达谱。应用MTT法和克隆形成实验检测miR-125a-5p对喉癌Hep2细胞增殖的影响。结果:喉癌组织中的let-7f-5p、miR-10a-5p、miR-125a-5p、miR-144-3p、miR-195-5p、miR-203等6个miRNA在基因芯片以及qRT-PCR中表达均显著下调。与对照组相比,转染miR-125a-mimics组的喉癌Hep2细胞增殖能力受到抑制,而转染miR-125a-inhibitor组Hep2细胞增殖能力增强。结论:基因芯片与qRT-PCR结果一致;喉癌与正常喉黏膜之间存在明显的miRNA差异表达,这些miRNA的差异性表达可能与喉癌的发病、侵袭等相关。miR-125a可以抑制喉癌Hep2细胞的增殖,可能作为喉癌生物治疗的新靶点。
AIM: To investigate the differential microRNA expression profiles between laryngeal cancer and adjacent normal laryngeal mucosa. METHODS: Forty two pairs of laryngeal cancer tissue and adjacent normal laryngeal mucosa tissue were collected. Ten pairs of samples were used for determining microRNA expression by the method of miR- NA microarray chip. Data analysis was performed to find out the significant differential microRNA expression profile in la- ryngeal cancer, and the difference was verified by quantitative real-time PCR (qRT- PCR) analysis on another 32 pairs of samples. Methyl thiazolyl tetrazolium (MTT) assay and colony-forming assay were used to analyze the proliferation of Hep2 cells induced by miR-125a-Sp. RESULTS: Both miRNA microarray and qRT-PCR showed that the expression of let-7b 5p, miR-10a-5p, miR-125a-5p, miR-144-3p, miR-195-5p and miR-203 was down-regulated in laryngeal cancer tissues. miR-125a-Sp suppressed the proliferation of Hep2 cells. CONCLUSION: The results of microarray are accordant with those of qRT-PCR. Significant difference of miRNA expression profiles between laryngeal cancer and adjacent normal laryn- geal mucosa indicates that miRNAs may play a role in carcinogenesis and progression of laryngeal cancer, miR-125a-5p in- hibits the proliferation of Hep2 cell, indicating a novel therapeutic target against laryngeal cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第1期86-92,共7页
Chinese Journal of Pathophysiology
基金
广东省科技计划(No.2011B031800148)
2011年第二批省级财政产业技术研究与开发专项资金(No.Z012011254)
关键词
喉肿瘤
微小RNA
微阵列芯片
细胞增殖
Laryngeal neoplasms
MicroRNA
Microarray chip
Cell proliferation