高压电烧伤大鼠血清CD44变化及乌司他丁干预作用

CD44 Expression Changes Induced by Experimental High-Voltage Electrical Burns in Rat and the Therapeutic Effects of Ulinastatin

目的了解大鼠高压电烧伤后血清CD44变化,探讨乌司他丁(UTI)对其的干预效果。方法将180只清洁级大鼠按随机数字表法分为对照组、电伤组和治疗组,每组60只,各组每时相点10只。电伤组和治疗组大鼠用调压器及实验变压器制成高压电烧伤模型,对照组接相同装置但不通电致假伤。于伤前15 min及伤后5 min、1 h、2 h、4 h、8 h,大鼠开胸后在直视下心脏采血,分离血清,用ELISA双抗体夹心法检测血清CD44含量。对数据行两因素析因设计的方差分析和LSD检验。结果①对照组和电伤组大鼠血清CD44含量的比较:电伤组CD44含量高于对照组(主效应F=882.901,P<0.01);电伤组CD44含量受伤后时间变量影响(主效应F=81.459,P<0.01),伤后5 min～8 h各时相均高于本组伤前值(P<0.01),且呈逐渐增高趋势。②电伤组和治疗组大鼠血清CD44含量的比较:治疗组CD44含量低于电伤组(主效应F=219.597,P<0.01);治疗组CD44含量受伤后时间变量影响(主效应F=155.774,P<0.01),治疗组伤后5 min～8 h各时相均高于本组伤前值(P<0.01)。结论高压电烧伤引起大鼠血清CD44含量升高,而UTI能降低高压电烧伤后的大鼠血清CD44含量。

Objective To explore the influence of high-voltage electricity on CD44 expression in rats＇ blood serum and the therapeutic effects of Ulinastatin （UTI）. Methods 180 SD rats were randomly divided into three groups, control group, electrici-cal bum group（burn group） and UTI-treated group（ treatment group） and each group contained 60 rats. 60 rats in each group were divided into 6 sub-groups and each sub-group included 10 rats. Voltage regulator and transformer was used o make high- voltage electricity bums（HEB） animal model. The above equipment without electricity was use to treat the rats of the control group. The collection of cardiac blood and the preparation of the serum were performed at 15 minutes before HEB ,5 minutes lat-er, 1 hour later,2 hours later,4 hours later and 8 hours later. The concentration of CD44 was assayed with ELISA. And the exper-imental data were analyzed statistically with variance analysis and LSD test. Results ①The comparison of CD44 expression be-tween the control and bum group ： the expression in bum group was increased compared with that in control group（ F = 882. 901, P 〈0.01 ） ,the increased CD44 expression in bum group was in a time-dependent manner and the expression at 5 minutes to 8 hours after the burn was higher than that before the bum（ F = 81. 459 ,P 〈 0.01 ）. ②The comparison of CD44 expression between the burn and treatment group：the expression in treatment group was reduced compared with that in burn group （ F = 219. 597, P 〈 0.01 ）. The change of the CD44 expression in treatment group was linked with time duration and the expression at 5 minutes to 8 hours after the bum was higher than that before the bum （ F = 155. 774, P 〈 0.01 ）. Conclusion HEB up-regulated the CD44 expression in blood serum of rats, and UTI reduced CD44 expression in blood serum of rats after HEB.