高血压MMP-9基因多态性与心脏及血管重构的关系

Relationship between matrix metalloproteinases-9 gene polymorphism and the cardiovascular remodeling in hypertension patients

目的研究基质金属蛋白酶基因多态性与高血压患者心脏及血管重构的关系。方法高血压患者53例,健康对照组30例。采用心脏彩色多普勒超声检测左心室舒张末期室间隔厚度(IVSTd)、舒张末期左心室后壁厚度(LVPWTd)、颈动脉内膜中层厚度(IMT)。测量患者上下肢血压并计算踝臂指数(ABI)。采用多聚酶链式反应结合限制性内切酶法检测基质金属蛋白酶-9(MMP-9)基因的C-1562T基因多态性。结果 MMP-9基因的C-1562T多态性与高血压的发生发展无相关性(P>0.05);携带T等位基因的高血压人群的IVSTd、LVPWTd和颈动脉IMT较不携带T等位基因高血压人群明显增厚(P<0.05);携带T等位基因的高血压人群均较不携带T等位基因高血压人群的ABI低;高血压患者的C-1562T基因多态性与IVSTd、LVPWTd和颈动脉IMT呈正相关(P<0.05),与ABI呈显著负相关(P<0.05),高血压患者年龄愈高其ABI愈小。结论 MMP-9的TC基因型是影响高血压患者心脑及血管重构的重要因素之一。

Objective To explore the relationship between matrix metalloproteinases-9 gene polymorphism and the cardiovascular remodeling in hypertension patients. Methods 53 hypertension patients were randomly selected. 30 healthy people were selected as the control group. Ventricular end-diastolic interventricular septum （IVSTd）, end-diastolic left ventricular wall thickness（LVPWTd） and carotid intima media thikness（IMT） were determined by Doppler ultrasonography. The blood pressure of upper and lower limbs was measured and the ABI was calculated. The C-1562T gene polymorphism of MMP-9 was measured by polymerase chain reaction combined with restriction enzyme digestion. Results MMP-9 gene polymorphism was not associated with the onset and development of hypertension （ P 〉 0.05 ） ; IVSTd, LVPWTd and IMT of hypertension patients who carried the T alleles was thicker than that of the hypertension patientswho didn＇t carry the T alleles（P 〈0.05） ;ABI of hypertension patieints who carried the T alleles was lower than that of the patients who didn＇ t carry the T alleles;The C-1562T gene polymorphism of the hypertension patients was positively correlated with the IVSTd, LVPWTd and IMT（ P 〈 0.05 ）, and was negatively correlated with the ABI（ P 〈 0.05 ）. Age had negative correlations with the ABI in the hypertension patients. Conclusion C-1562T gene polymorphism of MMP-9 gene is one of the important factors that effect the cardiovascular remodeling in the hypertension patients.