犬接种鼠疫菌后的血清鼠疫F1抗体及IgM动态

Dynamic of serum plague F1 antibody and IgM in dogs inoculated with Yersinia pestis

目的通过覌测犬接种鼠疫菌后鼠疫F1抗体及其IgM动态,推算发生动物鼠疫流行的时间和地点。方法应用ELISA夹心法检测犬鼠疫F1抗体,捕获法检测IgM,两种方法同步检测接种鼠疫菌EVparis株活菌的犬血清。结果犬接种鼠疫菌后第5d,抗鼠疫F1抗体和IgM都出现了阳性反应,IgM显色和滴度第7～9d达到高峰,第15～22d开始快速下降,第46d以后降到阳性标准以下;F1抗体在接种鼠疫菌后第38d显色和滴度达到高峰,第78d仍保持在OD450nm2.700以上和滴度1∶210左右。结论根据ELISA检测犬鼠疫F1抗体和IgM的结果,可推算动物间鼠疫流行的时间和范围,为及时实施防控措施提供参考资料。

The dynamic of plague F1 antibody and IgM of dogs inoculated with Yersinia pestis was surveyed to predict the time and place of animal plague. Both capture ELISA and sandwich ELISA were used synchronously to detect plague anti- body of dogs inoculated with EV_paris strains of viable Yersinia pestis. And the capture ELISA and sandwich ELISA were used to detect IgM and F1 antibody, respectively. Results indicated that both IgM and F1 antibody showed positive reactions on the 5th day after inoculation. The IgM color and titers reached a peak on the 7th-9th day, declined rapidly on the 15th-22nd day, and declined below positive criteria on the 46th day. The F1 antibody color and titers reached a peak on the 38th days after inocula- tion and remained above 2. 700 of OD_450nm and 1 ： 21^10 of titers until the 78th day. Based on the results of the ELISA for detec- ting F1 antibody and IgM of dogs, the timing and scope of animal plague epidemic could be predicted and the reference informa- tion for the timely implementation of control measures could be provided.