人乳头瘤病毒基因型在宫颈细胞病变中的分布

Prevalence of human papillomavirus genotype in cervical lesion

目的探讨人乳头瘤病毒(HPV)感染在宫颈病变中的分布及其与宫颈病变之间的关系。方法用刷拭子法对1591例门诊就诊妇女采集宫颈细胞,用PCR-膜斑点杂交法进行HPV分型,薄层液基细胞学检查(TCT)观察宫颈细胞病理变化。结果在338例TCT检出宫颈病变的女性中,HPV和HR-HPV感染率分别高达68.64%和63.02%,HPV和HR-HPV感染率均随着宫颈病变级别递增而升高,差异有统计学意义(P<0.05)。除HPV51外,其它HR-HPV基因型和HPVCP8304在不同宫颈疾病组中具有极显著差异(P<0.01)。年龄≤40岁妇女HR-HPV和LR-HPV感染的机率显著高于年龄>40岁妇女(P<0.05);年龄>40岁妇女HPV感染罹患宫颈相关疾病的机会显著高于年龄≤40岁妇女(P<0.01)。结论 HR-HPV与宫颈病变关系密切,适龄妇女作HPV筛查对预防宫颈癌具有重要意义。

The human papillomavirus （HPV） is a causative agent in the spectrum of cervical lesions. In this study, we explored the prevalence of HPV genotypes and the relationship between HPV infection and cervical pathology. Cervical cells were collected from 1 591 outpatient women with cervical lesion. HPV was genotyped by PCR, membrane-based hybridization, and the pathological changes of cervical cells were observed by Thinprep cytologic test. Our resulted showed that the total HPV infection rate was 38. 90% and the high-risk HPV genotype （HR-HPV） was 29. 47%. Among 338 patients suffered from cer- vical intraepithelial neoplasia, HPV and HR-HPV infection rate were 68.64% and 63.02 %, respectively. The prevalence of HPV infection was significantly higher among women with high grades squamous intraepithelial lesion （HSIL） than those with low grades squamous intraepithelial lesion （LSIL） （P〈0.01）. Furthermore, the prevalence of HR-HPV and LR-HPV were significantly higher among women below 40 years old than those above 40 years old （P 〈0.01）. But the risk of suffering from cervical intraepithelial neoplasia among women above 40 years old was significantly higher than that in women below 40 years old （P〈0.01）. We concluded that HPV genotyping could help to predict the high risk of cervical cancer, which will be benefi- ted for early diagnosis and therapy.