摘要
目的比较实时荧光PCR法与酶联免疫吸附试验(ELISA)在发热伴血小板减少综合征病例检测中的差异。方法对143例2011年河南省发热伴血小板减少综合征监测病例的急性期血清,分别应用实时荧光PCR法和ELISA-IgM方法进行检测,结果进行统计学分析。结果 143例病例经实时荧光PCR法检测,阳性率50.35%,经ELISA-IgM法检测,阳性率为37.76%,两种方法的检测有统计学意义(P<0.05)。对于间隔时间超过1w的病例,两种检测方法无统计学意义(P>0.05)。结论实时荧光PCR法更适合于发热伴血小板减少综合征病例的早期实验室诊断,ELISA方法可对发病1w以上病例的实验室诊断。
To compare real-time PCR and ELISA methods in detection of severe fever with thrombocytopenia syndrome (SFTS) cases, 143 acute sera of SFTS cases were collected in Henan Province, 2011. All the samples were detected by real- time PCR and ELISA-IgM, and the results were analysed in statistics. Result showed that the positive rate of real-time PCR in detection of 143 SFTS cases was 50.35 %, while that for ELISA-IgM was 37.76 %0. There was significant difference in results between real time PCR and ELISA (P〈0.05). However, there was no significant difference between results of these two methods if the sera sample collection was conducted one week after onset of illness. It's concluded that real-time PCR is a better method for early SFTS diagnosis in laboratory. Whereas ELISA could be used for laboratory diagnosis when sera sample col- lected one week after onset of illness,
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2013年第1期101-104,共4页
Chinese Journal of Zoonoses
基金
河南省医学科技攻关计划重大项目(2011010017)~~