摘要
目的：获得齐口裂腹鱼过氧化物酶体增殖物激活受体Y辅助活化因子1a（PGC-1a）cDNA序列，探讨其在齐口裂腹鱼肌肉组织中的表达规律。方法：根据斑马鱼基因PGC-1a序列设计引物，提取齐口裂腹鱼肌肉组织总RNA，经RT-PCR扩增PGC-1a仪基因序列；利用半定量RT-PCR分析齐口裂腹鱼PGC-1a基因在红肌和白肌中的mRNA表达特性。结果：获得齐口裂腹鱼PGC-1a基因序列2633bp，GenBank登陆号为JNl95738，其中ORF为2631bp，编码876个氨基酸残基，与同鲤科的斑马鱼、草鱼和金鱼的同源性较高，为88％～93％，但与哺乳动物和禽类如人、小鼠、大鼠、猪、牛和鸡的同源性较低，为49％～50％。在基础状态下，PGC-1a在齐口裂腹鱼红肌中的表达显著高于白肌，禁食可显著诱导PGC-1a在红肌和白肌中的表达水平。结论-首次克隆得到齐口裂腹鱼PGC-1a基因序列，其在红肌中的表达显著高于白肌中，禁食可显著诱导其在红肌和白肌中的表达，为研究PGC-1a在齐口裂腹鱼肌肉中的作用提供了理论依据。
Objective： To obtain the peroxisome proliferator-activated receptor 7 coactivator-1a（PGC-1a） gene se- quence of Schizothorax prenanti and study its expression in the red and white muscle tissue of S.prenanti. Meth- ods： PCR primers were designed according to Danio rerio PGC-1agene sequence. Using the total RNA of S.pre- nanti muscle tissue and RT-PCR method, we amplified PGC-1a gene sequence, and also analyzed the mRNA ex- pression level of PGC-1a gene in red and white muscle of S.prenanti using semi-quantitative RT-PCR. Results： A 2633 bp eDNA fragment（GenBank accession number： JN195738） of S.prenanti PGC-1a gene was obtained. The obtained nucleotide sequence covered an entire ORF of 2631 bp encoding 876 amino acids. Compared with zebraf- ish, grass carp and goldfish the homology of amino acid sequences were higher（88%～93% ）, but lower in some mammals and pouhry（49%～50%）. The gene expression level of PGC-1a gene in S.prenanti red muscle basal me- tabolism statu was significantly higher than that of white muscle（P〈0.05）, and PGC-1a was induced in S.prenanti red and white muscles under fasting condition. Conclusion： We firstly obtained the PGC-1a gene sequence of S. prenanti, and it＇s expression level in red muscle was significantly higher than that in white muscle, and PGC-1a was induced in red and white muscles by fasting. Our results will provide important theoretical basis for the role of PGC-1a on the muscle tissue of S.prenanti.
出处
《生物技术通讯》
CAS
2013年第1期71-75,共5页
Letters in Biotechnology
基金
国家自然科学基金(31201990)
西南民族大学中央高校基本科研业务费专项(10NZYZJ10)
