Annexin A2基因沉默对肺癌细胞转移能力的影响

RNA interference of Annexin A2 inhibits the metastasis of lung adenocarcinoma cells in vitro

目的:研究膜联蛋白A2(Annexin A2)基因沉默对体外培养的肺腺癌细胞系转移能力的影响。方法:体外培养4种转移潜能不同的肺腺癌细胞,采用Transwell迁移模型观察细胞的迁移能力。采用RT-PCR和Western blot分析分别检测细胞中Annexin A2 mRNA及蛋白的表达。应用RNA干扰技术沉默细胞中Annexin A2表达,观察Annexin A2基因沉默前后细胞迁移能力的变化以及哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)表达的变化。结果:Annexin A2在高转移潜能的肺腺癌细胞系中呈高表达状态(P<0.01)。Annexin A2基因沉默后,高转移潜能的肺腺癌细胞的迁移能力下降(P<0.05)。p-mTOR在高转移潜能的肺癌细胞中呈明显高表达(P<0.01),Annexin A2基因沉默后,细胞中p-mTOR表达明显下降(P<0.01)。结论:Annexin A2基因沉默显著抑制肺癌细胞的转移能力,Annexin A2对肺腺癌细胞转移能力的调控可能与其激活mTOR信号通路有关。

Objective：To investigate the effects of Annexin A2 on the metastasis of lung adenocarcinoma cells in vitro.Methods：Four lung adenocarcinoma cell lines of different metastatic potential were cultured in vitro.Cell migration,which indicats the metastatic potential,was measured with transwell migration assay.Annexin A2 expression was detected with reverse transcription polymerase chain reaction（RT-PCR） assay and Western blot analysis.The Annexin A2 expression was then silenced by RNA interference.The alteration in cell migration was measured with transwell assay and the change in mTOR expression was analyzed by Western blotting.Results：Annexin A2 expression was significantly higher（P0.01） in lung adenocarcinoma cells with high metastatic potential.Cell migration in the high metastatic potential cells was significantly inhibited（P0.05） by RNA interference of Annexin A2 gene.Expression of p-mTOR in the high metastatic potential cells was higher than in the low metastatic potential cells（P0.01） and decreased significantly after Annexin A2 gene was silenced（P0.01）.Conclusion：RNA interference of Annexin A2 inhibits the metastatic potential of lung adenocarcinoma cells in vitro.Annexin A2 might regulate the metastatic potential of lung adenocarcinoma cells through the activation of mTOR pathway.