SD鼠未成熟心肌细胞缺氧/复氧期c-fos,c-myc mRNA的表达

Expression of oncogene c-fos, c-myc in SD rat immature myocardium during hypoxia/reoxygenation

目的 研究未成熟心肌细胞缺氧 /复氧期间 c- fos,c-myc的表达 .方法 建立 SD大鼠未成熟心肌细胞原代培养及缺氧 /复氧模型 ,应用原位分子杂交技术 ,观察 c- fos,c- myc在心肌细胞核内的表达 .结果 未成熟心肌细胞在缺氧前 c-fos,c- myc m RNA无表达 ,对照 ( )组和 ( )组 c- fos,c- mycm RNA在缺氧 12 0 min开始表达 ,复氧 6 0 min表达程度进一步升高 .牛磺酸保护组 ( 组 )在缺氧 12 0 min时 c- fos,c- mycm RNA为阴性表达 ,在复氧 6 0 min时仅有弱阳性表达 .结论 c- fos,c- myc m RNA表达程度与心肌细胞损伤程度以及心肌细胞内游离钙浓度的变化密切相关 ,c- fos,c- myc基因表达可能对心肌细胞的损伤。

AIM To study the expression and significance of oncogene cfos, cmyc mRNA in hypoxia and reoxygenation of immature myocytes. METHODS A model of original cultural myocytes of neonatal SD rat and hypoxiareoxygenation injury of immature myocytes was established. By in situ hybridization methods, we studied the expression of oncogene cfos, cmyc mRNA in hypoxia and reoxygenation injury of immature cardiac myocytes. RESULTS The cfos, cmyc mRNA of immature myocytes were negative expressionbefore hypoxia. Group I and Ⅱ showed positive expression at 120 min after hypoxia. The expression of cfos,cmyc mRAN was strong at 60 min after reoxygenation. In group Ⅲ, the expression of cfos, cmyc mRNA was negative at 120 min after hypoxia. The cfos, cmyc mRNA expressed slightly only at 60 min after reoxygenation in group Ⅲ. CONCLUSION Expression of cfos and cmyc mRNA may be closely related to damage and repairing of myocardial cell as well as intracellular free Ca 2+ concentration. The expression of cfos, cmyc mRNA might act as modulators of the procedure of injury, repairing or and proliferating of cardiac myocytes.