间断给氧对兔体外循环中肺损伤的保护

Protective effects of intermittent ventilation against lung injury during cardiopulmonary bypass

目的 研究间断给氧对兔体外循环 ( CPB)中肺的保护作用 ,并探讨其机制 .方法 采用兔 CPB模型 ,CPB中间断给氧 ,测定肺组织的丙二醛 ( MDA ) ,髓过氧化物酶( MPO) ,肺组织血管通透性 ( EB值 )及湿干质量比 ( W/ D) ,并观察肺组织超微结构的变化 .结果 间断给氧组肺组织MDA含量 ( 81± 5 ) mol.kg- 1 明显低于 CPB组 ( 139± 17) mol.kg- 1 ,P<0 .0 1.MPO含量 ( 6 .3± 1.9)× 10 3U.kg- 1 明显低于 CPB组 ( 9.4± 0 .6 )× 10 3U.kg- 1 ,P<0 .0 1.血管通透性( EB值 ) ( 1.9± 0 .2 )明显低于 CPB组 ( 3.2± 0 .0 7) ,P<0 .0 5 .W/ D值 ( 8.7± 0 .8)明显低于 CPB组 ( 11.3± 2 .3) ,P<0 .0 5 .结论 CPB期间存在肺缺血再灌注损伤 ;间断给氧可减轻肺损伤 。

AIM To study the protective effects and mechanism of intermittent ventilation aganist lung injury during cardiopulmonary bypass (CPB) in rabbits. METHODS CPB models were set up with living rabbits by intermittent ventilation. MDA, MPO content in lung tissue was determined. Permeability of vascular (Evan's blue value) and ratio of wet to dry lung tissue were tested. The ultrastructure of lung was observed. RESULTS Compared with CPB group, MDA content of lung tissue in intermittent ventilation group (81±5) mol·kg -1 was significantly lower (139±17) mol·kg -1 , P<0.01; MPO content (6.3±1.9)×103 U·kg -1 also significantly lower (CPB: 9.4±0.6)×103 U·kg -1 , P<0.05; EB value (1.9±0.2) lower (CPB: 3.2±0.07), P<0.05 and ratio of wet/dry lung tissue (8.7±0.8) lower (11.3± 2.3 ), P<0.05. CONCLUSION There is ischemic/reperfusion injury during CPB and intermittent ventilation has protective effects.