降钙素基因相关肽对缺氧状态下培养的血管内皮细胞的影响

Effects of calcitonin gene related peptide on cultured anoxic human umbilical vein endothelial cells

目的 观察不同浓度的降钙素基因相关肽 ( CGRP)对缺氧状态下人脐静脉血管内皮细胞的影响 .方法 用培养的人脐静脉血管内皮细胞建立缺氧模型 ,观察血管内皮细胞在缺氧状态下细胞膜流动性 ,5 1铬释放率 ,台芬蓝摄取率和细胞内钙 ,镁含量的变化以及 1× 10 - 9,1× 10 - 8,1× 10 - 7mol.L- 1 CGRP对血管内皮细胞的影响 .结果 1× 10 - 9,1×10 - 8,1× 10 - 7mol.L- 1 CGRP可降低缺氧 12 0 min时细胞膜流动性 ,5 1 铬释放率 ,台芬蓝摄取率及减少细胞内镁的丢失 ,1× 10 - 8mol.L- 1 CGRP的效果最好 .结论 CGRP对血管内皮细胞缺氧损伤具有直接保护作用 。

AIM To investigate the effects of different concentrations of calcitonin gene related peptide (CGRP) on cultured anoxic human umbilical vein endothelial cells. METHODS An anoxia model was established by using cultured human umbilical vein endothelial cells. The endothelium membrane fluidity, 51 Cr release rate, uptake rate of trypanblue and intracellular calcium and magnesium contents were measured before and after 120 min anoxia and the effects of 1×10 -9 , 1×10 -8 and 1×10 -7 mol·L -1 CGRP on anoxic endothelial cells were studied. RESULTS The endothelium microviscosity of membrane fluidity (η) and 51 Cr release rate significantly increased and contents of endothelium magnesium decreased and uptake rate of trypanblue and contents of endothelium calcium remained unchanged after 120 min anoxia. CGRP at concentration of 1×10 -9 , 1×10 -8 and 1×10 -7 mol·L -1 was able to reduce the endothelium microviscosity of membrane fluidity (η) and 51 Cr release rate and the loss of magnesium after 120 min of anoxia while CGRP at a concentration of 1×10 -8 mol·L -1 had the best effects. CONCLUSION CGRP might lessen the damage of anoxic endothelial cells in a concentrationdependent manner, suggesting that CGRP may directly protect the endothelial cells afflicted with anoxic injury, which is related with its concentration.