高效液相色谱法测定金太子胶囊中人参皂苷Rg1&Re的含量

Determination of Ginsensides Rg1&Re Contents from Tasly Man Capsule by HPLC

目的建立金太子胶囊人参皂苷Rg1、Re的含量测定方法。方法优化色谱条件，确定采用Agilent（4．6mm×250mm，5um）色谱柱，以乙腈：水（21：79）为流动相，流速为1．0mL·min-1；检测波长为203nm；柱温30℃。结果结果表明，人参皂苷Rg1在0．0619-0．516mg·mL-1范围内呈良好线性关系（γ=0．9997），人参皂苷Re在0．0493-0．4944mg·mL-1范围内呈良好线性关系（1：0．9997）；人参皂苷Rg1、人参皂苷Re的回收率分别是100．25％、98．42％（n=6）。且分离度良好（达2．7左右）。结论本方法准确可靠，重复性和适用性良好，可以适用于金太子胶囊中人参皂苷Rg1＆Re的测定。

Objective To develop a HPLC method for determination of ginsenosides Rg1＆ Re from Tasly Man CapsμLe. Methods The seporation was performed on Agilent （4.6 mm×250 mm, 5 μm） column eluted with the mobile phase consisting acetonitrile and water solution （21：79） at the flow rate of 1.0 mL.min-1. The detection wavelength was 203 nm and the column temperature was 30℃. ResμLt Ginsenosides Rg1 showed good linearity （7=0.999 7） in the range of 0.061 9-0.516 mg-mL-1 and Ginsenosides Re showed good linearity （7=0.999 7） in the range of 0.049 3-0.494 4 mgomL1, and the average recovery of assay was 100.25%, 98.42% （n=6）. Conclusion The HPLC method was convenient, rapid, sensitive, accurate and reliable. It coμLd be used for determination of ginsenosides Rg1＆Re from Tasly Man CapsμLe.