耐亚胺培南铜绿假胞菌耐药性分析及耐消毒剂基因检测

Investigation on the antimicrobial resistance and disinfectant-resistant gene in imipenem-resistant Pseudomonas aeruginosa

目的了解临床分离的耐亚胺培南铜绿假单胞菌(IMPRPa)的分布和耐药性,以及携带耐消毒剂基因qacE△1-sull的情况。方法临床标本应用VITEK-2 compact全自动微生物鉴定仪进行鉴定及药敏试验,并对23株IMPRPa的耐消毒剂基因qacE△1-sull进行PCR扩增。结果本院IMPRPa主要来自新生儿科病房,其检出率为36.96%,高于儿科门诊及儿科病房(P<0.05);IMPRPa对氨基糖苷类、喹诺酮类药物敏感,对广谱青霉素类、头孢类以及磺胺类的复方新诺明等几乎全部耐药;23株IMPRPa有19株qacE△1-sull基因阳性,携带率为82.6%。结论 IMPRPa早产儿易感,对常用抗生素耐药率高,耐消毒剂基因qacE△1-sull携带率较高,存在抗消毒剂的可能性。

Objective To investigate the antimicrobial resistance and the condition of disinfectant-resistant gene（qacEDeltal and sull gene） in imipenem-resistant Pseudomonas aeruginosa（IMPRPa）.Methods The bacteria were cultured and isolated according to National Clinical Laboratory Operation Manual,and identified and tested by Compact VITEK-2.Polymerase chain reaction（PCR） was performed to detect disinfectant-resistant gene qacE△1-sull in 23 isolates of IMPRPa.Results The positive rate of the IMPRPa was the highest in the department of Neonatal ward in this hospital,with positive rate of IMPRPa for 36.96%.IMPRPa were all susceptible to Aminoglycosides and Quinolones agents,but almost all resistant to broad spectrum penicillins,Cephalosporins and Sulfonamides antibiotics.19 of 23 strains were qacE△1-sull gene positive,and the carrying rate was 82.6%.Conclusion Premature infant might be susceptible to IMPRPa.The resistance rates of IMPRPa to common antibiotic might be high and the resistance patterns could be wide.There might be with high positive percentage of qacE△1-sull gene in IPMRPa.