甘肃地区藏族丙型肝炎患者的HCV基因分型研究

Genotyping of HCV DNA in Tibetan nationality patients with hepatitis C in Gansu

目的了解甘肃地区藏族丙型肝炎患者的HCV基因分型特征。方法对HCV感染的患者样本用实时荧光定量PCR进行病毒载量的确定。对病毒载量大于103 copy/mL的样本用多重PCR进行HCV基因分型。结果通过对甘肃地区藏族HCV感染者的样本进行基因分型,发现该民族HCV感染以1b型(56.6%)为主,HCV感染2a型(23.3%)次之,再次为2c型(10.0%)和1c型(3.3%),未检测到3a型。结论甘肃藏族HCV感染以1b型为主,该研究为临床针对不同基因型HCV感染者的治疗提供了依据。

Objective To investigate hepatitis C virus（HCV） genotypes in Tibetan nationality of Gansu. Methods HCV load was tested by real-time quantitative PCR. Samples of different genotypes with viral load more than 10s copy/mL were analyzed by multiplex PCR. Results Tibetan ethnic group were mainly infected with HCV type lb（56.6%）, followed by type 2a（23.3%）, 2c （10.0%） and lc（3.3%）. However, type 3a could not be identified. Conclusion It might be with special feature that the genotypes of Tibetan nationality patients were mainly infected by type lb HCV. Results of the research could provide theoretical basis for the clinical care of HCV infected individuals with different genotypes.