尿沉渣分析仪红细胞质控液的制备与分析

Development and analysis of erythrocyte quality control material for urinary sediment analyzer

目的探讨尿沉渣分析仪红细胞质控液的制备方法。方法以0.05%、0.10%、0.15%、0.20%戊二醛浓度对洗涤红细胞进行全固定,以0.005%、0.010%、0.015%戊二醛浓度进行半固定,再分别用PBS和添加Na2SO4的PBS稀释至不同浓度,观察红细胞计数结果波动情况及质控液红细胞黏附尿沉渣分析仪计数板的情况。结果与UF-500i质控液相比,全固定与半固定红细胞质控液半年内的稳定性都很好,但全固定红细胞容易黏附计数板,Na2SO4则可减少细胞黏附;半固定红细胞不易黏附计数板,且低浓度红细胞以及高浓度醛固定对提高红细胞的稳定性有一定作用。结论半固定红细胞质控液比全固定红细胞质控液更适合临床应用。

Objective To develop erythrocyte quality control material for urinary sediment analyzer. Methods Washed fresh red blood cells（RBC） were totally fixed by glutaraldehyde with final concentrations of 0.05 %, 0.10%, 0.15 % and 0.20% respectively, while partially fixed with 0. 005% ,0. 010% and 0. 015% glutaraldehyde,which were both diluted at different proportion with PBS buffer and PBS buffer added with Na2 SO4 respectively. The number of RBC was measured by hematology analyzer,and the situation of RBC adhering on the counting chamber in urinary sediment analyzer was observed. Results Both total and partial fixed RBC were stable in half year. In partial fixed material, relatively higher glutaraldehyde concentration contributed to the stabilization of RBC. Compared with UF-500i quality control liquid, totally fixed erythrocytes were more likely to adhere on the counting chamber, however, it could be inhibited by Na2SO4. Partial fixed erythroeyte adhering on counting chamber was not observed. Conclusion Partial fixation method for preparing RBC quality control material might be more suitable for clinical application than total fixation method.