整合子与多耐药铜绿假单胞菌的相关性研究

Relativity between integrons and multi-drug resistance of Pseudomonas aeruginosa

目的研究分离自烧伤患者的铜绿假单胞菌(Pa)整合子携带情况及其与多药耐药的相关性。方法对50株Pa采用K-B法进行药敏实验,简并引物扩增法扩增整合子,限制性片段长度多态性(RFLP)分析确定整合子类型。结果 50株Pa对22种抗菌药物的耐药率达90%以上;49株(98%)携带Ⅰ类整合子,未检出Ⅱ、Ⅲ类整合子。结论该院Pa临床菌株对β-内酰胺类、氨基糖苷类、喹诺酮类等抗菌药物耐药率较高,多药耐药性可能与Ⅰ类整合子有关。

Objective To investigate the types of integrons in Pseudomonas aeruginosa（Pa） isolated from patients with burn and to analyze the correlation between integrons and multi-drug resistance. Methods 50 strains of Pa were eollected and detected for antibiotics susceptibility by K-B method, for integrons polymerase chain reaction using degenerate primers,for classification of inte- grons by restriction fragment length polymorphism （RFLP） assay. Results The drug-resistance rates of 50 strains of Pa against 22 kinds of antibiotics were higher than 90%. Class I integrons were founded in 49 stains （98%）. Neither class Ⅱ nor class Ⅲ of in- tegrons were detected. Conelusion Pa might be with high resistance rates against β-1actams,aminoglycosides and fluoroquinolones, and class I integron could be closely associated with multi-drug resistance of Pa in the hospital.