人FKBP12基因的可溶性表达及活性研究

Expression and bioactivity of human FKBP12 gene

目的 获得具有生物学活性的hFKBP12 ,以筛选新型的促神经再生药物。方法 构建原核表达载体 pExSecI/hFKBP12 ,并在BL2 1(DE3) plysE菌株中进行可溶性高效表达。表达上清经CM Sepharosefastflow一步层析。 结果 获得电泳纯的hFKBP12。活性测定结果表明 ,纯化后的hFKBP12显示具有较强的肽基脯氨基顺反异构酶活性。

Aim To obtain bioactive hFKBP12 protein for screening novel neurotrophic drugs. Methods hFKBP12 gene was inserted into prokaryotic expression vector pExSecI and then expressed in soluble form with high efficiency in E.coli BL21(DE3)plysE host. Recombinant hFKBP12 protein were purified by one step CM Sepharose fast flow chromatography. Results Recombinant hFKBP12 protein,with a purity of homogenity, showed peptidylprolyl cistrans isomerase(PPIase)activity. Conclusion Recombinant hFKBP12 protein is bioactive just as it wild type's.