Bc-l2核酶诱导SMMC7721细胞凋亡与p16,p21蛋白表达的关系研究

Relationship between apoptosis induced with bcl-2 ribozyme and expression of p16, p21 in SMMC7721 cells

目的 观察bcl 2核酶对人肝癌细胞株SMMC 772 1细胞的作用 ,并检测 p16和 p2 1的表达情况。探讨bcl 2核酶在肝癌治疗中的意义。方法 经脂质体介导的方法 ,将PMTr neo (正向bcl 2核酶真核表达载体 )导入SMMC772 1细胞中。细胞克隆转移扩大培养后 ,采用TUNEL法、免疫组化技术结合图像分析 ,在检测SMMC772 1/PMTr neo细胞凋亡的同时 ,检测 p16 ,p2 1的表达。结果 与对照组相比较 ,在bcl 2核酶诱发SMMC772 1细胞发生凋亡的同时 ,p16和 p2 1基因的表达水平显著增高。结论 bcl 2核酶通过封闭bcl 2的表达可促进SMMC772 1细胞凋亡 ,同时伴发 p2 1和

Aim To observe the effect of bcl-2 ribozyme on SMMC7721 cells and the expression of p16, p21,so to investigate the role of bcl2 ribozyme in hepatocarcinoma treatment. Methods PMTrneo (sense bcl2 ribozyme eucaryotic expression vector) was introduced into SMMC7221 cells through lipofectin mediation. After cloning, TUNEL and IHC in combination with imaging analysis were used to simultaneously determine apoptosis and p16, p21 expressions of the SMMC7721/PMTrneo cells. Results Compared with control, in the course of apoptosis induced by bcl2 ribozyme p16 and p21 expressions rose significantly in SMMC7721 cells. Conclusion bcl2 ribozyme can promote SMMC7721 cell apoptosis by blocking bcl2 expression, which is accompanied by an increased p16 and p21 expressions. This result could be of value for studying hepatocarcinoma genesis and probing therapeutic procedure.