海岛棉一个受体类似蛋白基因Gbvdr3的启动子特征分析

Characterization of the promoter of a receptor like gene Gbvdr3in island cotton

通过染色体步移法(Genome walking)获得海岛棉一个受体类似蛋白基因Gbvdr3的启动子序列,并用PLACE软件对其进行序列分析。结果显示该启动子含有许多与抗逆和光调控相关的元件。将该启动子片段融合GUS报告基因进行拟南芥转化,对转化后代进行抗性筛选和PCR鉴定,共获得8株转基因植株。GUS染色表明,GUS基因主要在根部、花器官和种子中表达,因此推测Gbvdr3基因的启动子是一个组织特异性启动子。

In this study,the promoter region of a receptor like gene Gbvdr3 in Gossypium barbadense was obtained by genome walking,and the sequence was analyzed by PLACE software.The sequence analysis showed the promoter contained elements related to stress resistance and light regulation.The cloned promoter was fused to GUS reporter gene and was transformed into Arabidopsis.Eight transgenic plants were obtained by kanamycin sulfate screening and PCR method.GUS staining revealed the GUS expression was specifically detected in roots,flowers and seeds.The results indicated that the promoter of Gbvdr3 was a tissue-specific promoter.