鸭CYP7A1基因编码区C129T突变对肉质和脂肪性状的遗传效应分析

C129T mutation of CYP7A1 gene coding region and its genetic effects on meat quality and fatness traits in duck

采用PCR—SSCP法检测3个鸭群体CYP7A1基凶编码区87～246bp区域多态性．分析其多态性对肉质和脂肪性状的关联性。结果表明：金定鸭和兴义鸭群体中没有检测到多态性，全部为｜厂r基因型；樱桃谷鸭群体中检测到TT和TC2种基因型．TT基因型频率为0．8909，T等位基因频率为0．9454；序列比对发现，c．129C〉T沉默突变，表现为低度多态，处于Hardy—Weinberg平衡状态（P〉O．05），T-检验显示，TT基因型个体的肌内脂肪和血清总胆固醇显著低于TC基因型个体，其他指标在两个基因型问的差异均未达到鼹著水平。揭示等位基因C町能是脂肪沉积的有利等位基因，可作为提高肉鸭肌内脂肪的选择性标记。

The polymorphism of from 87 bp to 246 bp of CYP7A 1 gene coding region of three duck populations was detected by PCR-SSCP method, and then its association with meat quality and fatness traits were further analyzed. The results indicated that two genolypes （TT and TC） were observed in Cherry Valley duck. TT genotype and T allele frequencies were 0.8909 and 0.9454, respectively. c.129C〉T of silent mutation was found, which belonged to low polymorphism, and it was in accordance with Hardy-Weinberg equilibrium. The polymorphism hadn＇t been checked in Jingding duck and Xingyi duck. T-test revealed that intramuscular fat and serum total cholesterol with TI＂ genotype had significant lower than TC genotype. Other traits were no significant difference between two genotypes. These results suggest tha＇, callele might be advantage allele for fat deposition and could be used as a genetic marker for improving duck intramuscular fat.