虎尾轮根黄酮类化合物对小鼠肝脏功能影响

Effects of Flavonoids from Uraria crinita on Liver Function in Mouse

以小鼠为动物模型,在饲料中添加分离纯化虎尾轮根的黄酮类化合物,研究其对小鼠肝脏功能的影响。取体重为(20±2)g小鼠84只,随机分为6组,即:空白对照组,模型组,阳性对照组即联苯双酯组(150 mg/kg),以及虎尾轮黄酮高、中、低剂量组(450,300,150 mg/kg),连续给药21 d。以四氯化碳(CCl4)进行建模,采集小鼠血清及肝组织匀浆液进行T-AOC、ALB、TP、AST、ALT、MDA、SOD、GSH-Px等指标的检测;取小鼠肝脏进行组织切片观察。结果表明,(1)与模型组比较,虎尾轮黄酮各剂量组均能分别显著(P<0.05)或极显著(P<0.01)地提高小鼠SOD、T-AOC、GSH-Px、ALB、TP水平和降低AST、ALT、MDA水平;(2)各剂量组肉眼观察无明显的病理变化,对各试验组的肝脏组织显微结构进行比较,发现虎尾轮黄酮各剂量组对CCl4引起的肝损伤有不同程度的恢复,保肝护肝作用明显。可见虎尾轮黄酮对小鼠的肝损伤有显著保护作用。

The isolated and purified flavonoids from Uraria crinita were added in the diets of the mouse which were as animal models.The effects of the flavonoids on mouse liver function was studied.Eighty four mouse,weight（20±2） g,were randomly divided into 6 groups,blank control group,model group,the positive control group that bifendate group（150 mg/kg）,high dose of flavonoids group（450 mg/kg）,medium dose of flavonoids group（300 mg/kg） and low dose of flavonoids group（150 mg/kg）.All flavonoids groups of mouse were fed with the chemical for 21 continuous days.Carbon tetrachloride（CCl4） was used in the model group,and the mouse serum and liver tissue homogenates were used to the detection of the TAOC 、ALB、TP、AST、ALT、 MDA、SOD and GSHPx. The livers of mouse were removed to produce the slice.The results showed as follows：（1） The SOD,TAOC, GSHPx, ALB and TP level in the mouse treated with the all flavonoids group were significantly（P0.05） or extreme significantly（P0.01） higher than that in the mouse of the model group,and the AST,ALT and MDA level were lower.Flavonoids Uraria crinita could obviously affect on the repair of chemical liver.（2） Compared to the mouse in the model group,the liver form of the mouse treated with different doses of flavonoids from Uraria crinita had no significantly pathological changes observed with eyes.In the microstructure, the pathological damage was minor with varying degrees of recovery.So the flavonoids from Uraria crinita had a significantly protective effect on mouse liver injury induced by carbon tetrachloride.