枯萎病菌胁迫下黄瓜叶片蛋白质组分析

Proteomic Analysis of Cucumber Leaves Differentially Expressed Proteins Induced by Fusarium Wilt-Susceptible and Resistant Cucumber

以黄瓜感枯萎病品系1900和抗枯萎病转基因品系1905为试材,采用灌根法接种枯萎病菌分生孢子悬浮液,48h后提取叶片蛋白质,采用差异蛋白质双向电泳技术研究枯萎病胁迫下黄瓜叶片蛋白质组的变化。结果表明:黄瓜幼苗在枯萎病菌胁迫下,感病和抗病品系的双向电泳图谱存在一定差异。通过质谱分析,共鉴定出3-磷酸甘油醛脱氢酶、景天庚酮糖-1,7-二磷酸酶、木葡聚糖内转糖苷酶、聚光复合体叶绿素a/b结合蛋白、假拟蛋白和5类表达差异的蛋白点。这些植物代谢中产生的能量和一些小分子可能激发了黄瓜抗枯萎病基因的表达。

Fusarium wilt is a significant disease problem in cucumbers affecting yield and fruit quality globally. Modern molecular methods, such as transformation and protomatics, are marching their ways in solving this problem. Fusarium wilt- susceptible cucumber 1900 and -resistant transgenic line 1905 were irrigated with 1.0×10^6·mL^-1 Fusarium oxysporum. 48b later, their leaves were harvested for employing two-dimensional gel electrophoresis to analyze the proteome differences between the two types of cucumber. Five differentially expressed proteins were identified by MS, including glyeeraldehyde-3-phosphate debydrogenase, sedobeptulose-l,7- bisphosphatase, xyloglucan endotransglyeosylase, ehlorophy II a-b binding protein 2 and hypothetical protein. These metabolism-related enzyme and small molecules may stimulate the expression of Fusarlum wilt- resistant gene, and the results provide new clues for future work on anti-disease cucumber studies.