大环内酯类药物对化脓隐秘杆菌防突变浓度的研究

Mutant Prevention Concentrations of Macrolides Antibiotics aganist Arcanobacterium Pyogenes

测定5种大环内酯类药物对化脓隐秘杆菌的防突变浓度(MPC),为临床合理用药奠定理论基础。采用肉汤法富集化脓隐秘杆菌使菌液浓度为1010CFU.mL-1,采用琼脂二倍稀释法测定红霉素、阿奇霉素、泰乐菌素、乙酰螺旋霉素和替米考星对化脓隐秘杆菌的MPC,计算选择指数(SI)。结果表明:各药的MPC和SI值分别为:红霉素1.02μg.mL-1、25.5;阿奇霉素0.64μg.mL-1、16;泰乐菌素0.2μg.mL-1、10;乙酰螺旋霉素0.8μg.mL-1、16;替米考星1.02μg.mL-1、20.4。泰乐菌素的MPC及SI值最低,不易产生耐药突变株,建议临床选择泰乐菌素治疗化脓隐秘杆菌引起的感染。

The mutant prevention concentrations （MPC） of five macrolides antibiotics against A. pyogenes were measured in this study. The MPCs and select index（S1） of Erythromyein, Azithromycin, Tylosin, Acetylspiramycin and Timicosin against A. pyogenes were tested by the agar dilution method. The results were as following： the MPCs of Erythromycin, Azithromycin, Tylosin, Aeetylspiramycin and Timicosin against A. pyogenes were 1.02μg·mL^-1, 0.64μg·mL^-1 0.2μg·mL^-1, 0.8μg·mL^-1 and 1.02 μg·mL^-1 respectively; while the Sis were 25.5, 16, 10, 16 and 20.4, respectively. These results showed that the MPC and SI of Tylosin were the lowest among these antibiotics, which suggested that Tylosin would be the most effective way in curing the infection caused by A. pyogenes.