鹅源鸭1型甲肝病毒的分离与鉴定

Isolation and Identification of Duck Hepatitis Virus A type 1 with Goose-origin

2012年广东省某鹅场发生一种以肝脏出血为主要病变特征的传染病。从病死鹅肝脏中分离到1株病毒(命名为GD-01),该病毒能在96h内致死10日龄番鸭胚。人工感染8日龄雏番鸭后,濒死前出现角弓反张,病变为肝脏出血。应用鸭1型甲肝病毒特异性引物对GD-01进行RT-PCR检测,可扩增到约199bp目的条带。经RT-PCR鉴定和遗传进化分析证实该病毒分离株为鹅源鸭1型甲肝病毒。

A disease with gosling liver bleeding as the main pathological syndrome occurred in Guangdong Province in 2012. A virus strain named GD-01 was isolated from a dead gosling, which could cause death on the duck embryo within 96 hours. 8 days-old Muscovy ducklings infected with GD-01 showed the opisthotonos and liver bleeding before deuth. GD-01 was characterized by RT-PCR using specific primers for duck hepatitis virus A type 1, and an 199 bp fragment was amplified. Genetic analysis showed that the isolated strain belonged to duck hepatitis virus A type 1.