基于SSR标记的杂交籼稻主要不育系遗传多样性分析

Genetic Diversity Analysis of Main Male Sterile Lines for Indica Hybrid Rice Based on SSR Markers

采用SSR标记对我国杂交籼稻15个主要不育系进行遗传多样性分析。结果表明,在水稻基因组中平均分布的99个SSR位点其中71个SSR位点具有多态性,多态性位点率71.7%。71个SSR标记共检测到175个等位基因,每个SSR位点检测到2～5个,平均为2.47个等位基因,He平均为0.716,PIC平均为0.393。供试不育系在遗传距离0.693处聚为两大类群,广占63S单独聚为一类;另一大类包括其余4个光温敏核不育系和所有的三系不育系。聚类结果与系谱分析基本吻合。

Genetic diversity was analyzed among 15 main male sterile lines of indica hybrid rice from China with 99 pairs of SSR marker evenly distributed in the 12 chromosomes of rice.The results indicated that 71（71.1%） pairs of SSR marker showed polymorphism.There were 171 alleles detected with those 71 pairs of SSR marker.The number of alleles for per locus ranged 2-5 with an average of 2.47.The average of He was 0.716 and average of PIC was 0.393.The 15 main male sterile lines were divided into two groups at genetic distance 0.693.The sterile line Guangzhan 63S could be grouped alone.Another group was composed of four photo-thermo-sensitive genic male sterile lines and all main three-line male sterile lines.The clustering results based on SSR analysis was basically consistent with the pedigree of 15 main male sterile lines.