秦岭羚牛性别鉴定的初步研究

Primary Study on Sex Identification of Takin

根据牛的Sry基因核心序列设计合成1对引物F1、R1。应用PCR技术对羚牛(TAKIN)Sry基因进行扩增,结果在羚牛雄性样本扩增出1条带(230 bp),而在雌性样本未见扩增带,显示了Sry基因的性别特异性,应用这1对引物对60只未知性别的羚牛组织样本进行了性别鉴定,结果雄性14个,雌性46个。该试验为羚牛种群性别比率及其种群动态变化机制研究提供了资料。

In order to study mechanism of sexing, the PCR technique was used to amplify the Sty gene of takin. A pair of primers （F1 and R1） was designed and synthesized according to the motif of cattle SRY gene. As a results, a DNA fragment （230 bp） was amplified from male takin, none DNA fragment was amplified from female takin, which indicated the Sry gene was sex specificity. Using these primers, 60 sex-unknown samples of takin were successfully sexed（ 14 males and 46 females）. The results may con- tribute to study the dynamic mechanism and sex ratio of the takin population.