毛子草提取物体外抗氧化活性初步研究

Preliminary Investigation in Antioxidant Activity in vitro of Extracts from Incarvillea argute

采用80%乙醇和蒸馏水对毛子草进行提取,乙醇提取物加水分配后依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取相应的提取物和水层部分。水提部分经醇沉、干燥后得到毛子草粗多糖。再采用Fe3+还原能力、水杨酸法和二苯代苦味基肼自由基(DPPH.)法,比较毛子草不同提取物的抗氧化活性。结果表明:以上提取物均对Fe3+具有较强的还原能力,其中乙酸乙酯萃取物的还原力最强,但弱于对照品Vc;除粗多糖、水提取物和正丁醇提取物有较弱的清除羟基自由基活性外,其余各提取物基本都没有清除羟基自由基活性;毛子草各提取物对DPPH自由基有较强的清除作用,以乙酸乙酯提取物清除能力为最强,但各提取物的清除能力均较Vc稍弱。说明毛子草各提取物都有一定的抗氧化活性,尤其是抗DPPH的活性最佳。

The antioxidant activities in vitro of different extracts from lncarviUea argute were detected by the deoxidization Fe3＋ reaction system, salicylic acid method as well as DPPH free radical （DPPH · ） method in the present work. Extracts were obtained by treating material of L argute with 80% aqueous ethanol, subse- quently, were extracted with petroleum ether, chloroform, ethyl acetate and n-butanol. Different extracts were obtained respectively from corresponding extracts and water layer extract. Raw polysaccharide was oh- tained from water extract using ethanol precipitation. All of the extracts had strong deoxidization ability of Fe3~ , in which ethyl acetate extract showed the high deoxidization ability, but weaker than that of Vitamin C. The raw polysaccharide, n-butanol extract and water extract had weak scavenging effect on the hydroxyl free radical, while other extracts had no activity. The strong anti-DPPH free radical activity was observed from the different extracts, and ethyl acetate extract demonstrated the best scavenging effect on DPPtt free radical. Therefore, the extracts from L argute had antioxidant activities in vitro, especially anti-DPPH free radical effect, which provided scientific evidences for Miao Minzu medicine with anti-inflammatory effects.