贵州梵净山藤茶及其近缘种广东蛇葡萄挥发性成分比较

Extraction and Determination of Volatile Constituents from Leaf of Ampelopsis grossedentata and Ampelopsis cantoniensis

采用水蒸气蒸馏乙醚浸提藤茶(显齿蛇葡萄)和广东蛇葡萄挥发性成分,用GC-MS对挥发性成分进行分离鉴定,运用峰面积归一化法测得各成分的百分含量,并对两种植物挥发性成分进行比较,旨在为优质藤茶品种选育提供依据。结果表明:分离出藤茶(显齿蛇葡萄)挥发性成分38个,鉴定出28个组分,含量占挥发油的73.68%,其主要成分为烷烃类、醛类、有机酸、醇类及甾醇类等化合物;分离出广东蛇葡萄挥发性成分34个,鉴定出27个组分,含量占挥发油总量的79.41%,主要成分为烷烃类、有机酸类、酚类、醇类及甾醇类化合物。虽然两种植物含量最高的成分均为穿贝海绵甾醇(≥30%),但其他高含量成分大多数不同。

In order to provide information for high quality tengcha breeding, the volatile constituents in leaves of Ampelopsis grossedentata and Ampelopsis cantoniensis in Mt. Fanjing were extracted with ethyl ether, sep- arated and identified by GC-MS, subsequently, the content of each component was determined by peak area normalization method in the present study. The results showed 38 volatile components were separated, and 28 components were identified from Ampelopsis grossedentata, which accounted for 73.68% of the total volatile oil. The main ingredients included paraffin, aldehydes, organic acids, aleohols and srerol. Thirty-four vola- tile components was separated, and 27 components were identified from Ampelopsis cantoniensis, which ac- counted for 79.41% of volatile oil, and the main ingredients were paraffin, organic acids, phenolic, alcohols and srerol. No deference in clionasterol content had been investigated between the two species, however, oth- er high-content constituents were different. Therefore, the volatile compositions from these species were obvi- ously different both in content and constituent.