肾康注射液对肾小管上皮细胞LLC-PK_1分泌型胶原的影响

The effects of Shenkang injection(肾康注射液) on the collagen Ⅳ of LLC-PK_1 renal tubular epithelial cells

目的 :探讨肾康注射液对肾小管上皮细胞 L L C PK1 分泌 型胶原 (Co )的影响。方法 :用细胞酶联免疫吸附法 (EL ISA ) ,以单味大黄注射液为实验对照组 ,检测肾康注射液对肾小管上皮细胞 L L C PK1 分泌Co 含量的影响。结果 :肾康注射液可以显著抑制肾小管上皮细胞 L L C PK1 分泌 Co 的含量 ,并呈剂量依赖关系 ;肾康注射液作用明显优于同等含量的单味大黄注射液。结论 :肾康注射液抑制肾小管上皮细胞 L L CPK1 分泌 Co 含量 ,是该中药复方延缓慢性肾功能衰竭进展的机制之一。

Objective:To study the effects of Shenkang injection(肾康注射液) on the collagenⅣ(CoⅣ) of LLCPK 1 renal tubular epithelial cell.Methods:Using enzymelinked immunosorbent assay(ELISA) to observed the effects of Shenkang injection on the CoⅣ of LLCPK 1 renal tubular epithelial cells,in contrast group treated with the injection that was extracted from Rheum officinale Baill.Results:CoⅣ of LLCPK 1 renal tubular epithelial cell was inhibited by Shenkang injection,and those actions were doesdependent,more remarkable than that of the injection that was extracted from Rheum officinale Baill.Conclusions:The mechanism of delaying chronic renal failure with Shenkang injection is achieved by inhibiting CoⅣ of LLCPK 1 renal tubular epithelial cell.