摘要
为了更简便高效地分析烟叶中游离茄尼醇的含量,建立了一种超高效液相色谱(UPLC)快速分析方法。将烟叶样品用甲醇超声提取10 min,经膜过滤直接进行超高效液相色谱分析;在1.7μm BEH C18色谱柱上用甲醇洗脱,在5 min内实现了茄尼醇的快速分离,并用电喷雾四级杆-飞行时间质谱(Q-TOF-MS)对m/z 613.57和631.58进行了定性分析。结果表明,茄尼醇的质量浓度范围为0.765～76.5μg/mL时,峰面积与质量浓度具有良好的线性关系,相关系数为0.99924,方法的检出限7.65 ng/mL,RSD为2.62%,平均回收率为91.1%。该方法适合于烟叶中游离茄尼醇含量的快速准确测定。
In order to determine the content of free solanesol in tobacco leaves quickly and efficiently, a rapid Ultra-high Pressure Liquid Chromatography （UPLC） method was developed. By ultrasonic extraction with methanol, the extracting solution was membrane filtered to analyze by UPLC. The extract was eluted with methanol onto a short 1.7 μm BEH C18 column, and solanesol was separated rapidly in 5 rain. According to the ion peaks of trdz 613.57 and 631.58, qualitative analysis was carried out by Electrospray Ionization-Quadropole Time-of-Flight Mass Spectrometry （Q-TOF-MS）. The results showed that the linearity range of the proposed method was 0.765-76.5μg/mL, the RSD% was 2.62%, and the average recovery rate was 91.1%. The method was appropriate for quick and sensitive determination of free solanesol in tobacco.
出处
《中国烟草科学》
CSCD
2012年第6期80-84,共5页
Chinese Tobacco Science
基金
贵州省烟草公司重点科技计划项目(2009-09)
